However, with only 20 samples per barcode, multiple barcode sets (batches) are required to address questions in robustly powered study designs. A barcoding approach allows for 20 unique samples to be pooled and processed together in one tube, reducing the intra-barcode technical variability. 4Division of Allergy and Immunology, Department of Pediatrics, University of Colorado School of Medicine, Children's Hospital Colorado, Aurora, CO, United StatesĬytometry by Time-Of-Flight (CyTOF) uses antibodies conjugated to isotopically pure metals to identify and quantify a large number of cellular features with single-cell resolution.3Department of Statistics, Oklahoma State University, Stillwater, OK, United States.2Department of Biostatistics and Informatics, Colorado School of Public Health, Aurora, CO, United States.1Department of Immunology and Microbiology, University of Colorado School of Medicine, Aurora, CO, United States.Baxter 1, Sidney Ogolla 1 †, Rosemary Rochford 1, Debashis Ghosh 2, Pratyaydipta Rudra 3 § and Elena W. Schuyler 1 ‡, Conner Jackson 1 ‡, Josselyn E.
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